Ok, Z490 Dark is in your signature
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Your GPU's should be in PCIe Slot 1 and Slot 2. This should split the main 16x lanes between both for 8x to both GPUs. Don't use PCIe slot 3 (the non-silver one).
Slot 2 shares lanes with Slot 4 (bottom slot). So, you probably can't have anything in Slot 4 because you probably need all 8 lanes in slot 2. I'd try removing anything in PCIe Slot 4.
GPU 1 in Slot 1. GPU 2 in Slot 2. NVMe in M.2 socket 1.
No U.2. Nothing in PCIe Slot 4. No GPU in PCIe slot 3 (this is 4 lanes from the PCH and will disable an M.2 slot, and probably won't work with a GPU).
And I think that should work...