What if you disable a few slots? Say kill 2-4 so only 1 is on, video on 5? Use a very low power device, like a network card.
Idea 1) the signal traces on one of the slots is messed up, either in the slot or on the PCB.
Idea 2) the PLX tanked.
Idea 3) the power circuit that drives those slots tanked. I've had problems with it being pissy if I mix power rails (I run dual single rail 80a supplies, each within 200mv of each other.)
Do you have a way to check the voltage on the slots, and a quality volt meter (fluke, bk, hp, ect)?
Some times stuff just fries. My first SR2 cooked its audio right off the bat. Windows and Linux would not see it anymore. I sold that board to my brother, and it still runs three years later.